Summary
Type: SPE
Application #: 300060
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection: For further analysis of the glycoalkaloids we recommend our HPLC column
NUCLEOSIL® NH2, mobile phase acetonitrile / 20 mM potassium dihydrogen
phosphate buffer (75 : 25, v/v), flow rate 0.7 ml / min
Conditions: Column conditioning:
2 column volumes methanol, then 2 column volumes dist. water
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
5 ml dist. water / methanol (6 : 4, v/v)
Elution:
15 ml methanol; evaporate to dryness and redissolve in 1 ml methanol
For products with high lipid contents such as potato chips, potato salad etc.
mix 1 ml of the concentrated eluate with 19 ml acetonitrile and pour through a
CHROMABOND® NH2 column (3 ml / 500 mg), which was conditioned with 2 column
volumes methanol and 2 column volumes acetonitrile. Wash with 5 ml acetonitrile
and elute with 10 ml methanol. Evaporate as above.
Authors: Macherey-Nagel
Source: LC Department